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A simple electronic volume cell sorter for clonogenicity assays.
J P Freyer1, M E Wilder, P L Schor
1Cell Biology Group, Los Alamos National Laboratory, New Mexico 87545.
Cytometry
|May 1, 1989
Summary
A new, affordable electronic volume flow cell sorter offers precise cell sorting for various applications. This cell sorter achieves high efficiency and speed without cytotoxicity, enabling accurate cell plating and analysis.
Area of Science:
- Biotechnology
- Cell Biology
- Instrumentation
Background:
- Cell sorting is crucial for biological research and diagnostics.
- Existing cell sorters can be expensive and complex.
- Need for accessible and user-friendly cell sorting technology.
Purpose of the Study:
- To describe the design and construction of a low-cost, single-parameter electronic volume flow cell sorter.
- To highlight the instrument's features for ease of operation and flexibility.
- To detail applications in cell-based assays and studies.
Main Methods:
- Construction using existing technology for affordability.
- Incorporation of features like aseptic plating, temperature control, and PC interface.
- Development of a remote, electrically activated fluidics system.
Main Results:
- The cell sorter operates at rates up to 2,000 cells/second with >80% efficiency.
- Demonstrated no cytotoxicity, ensuring cell viability.
- Successful routine use for precise cell plating in clonogenicity assays.
Conclusions:
- The developed cell sorter provides an accessible and efficient solution for cell sorting.
- Its features facilitate applications in cell survival studies, age compartment separation, and cloning assays.
- Offers advantages for precise cell manipulation in research settings.