TOXICITY OF CHLORPYRIPHOS CONTAINING FORMULATION AND HEAVY ELEMENTS (Cd, Pb) TO CHICKEN EMBRYOS

The phytotoxic effect of cadmium sulphate, lead acetate and chlorpyriphos containing insecticide Pyrinex 48 EC were investigated on chicken embryos on late developmental stage (day 19 of incubation). The eggs were injected by 0.1 ml amount of lead acetate (0.1%), of cadmium sulphate (0.01%), of Pyrinex 48 EC (chlorpyriphos, 480 g/l; 1%) and as a control 0.1 ml of bird-physiologic saline solution (0.75%). The treatments were performed on day 0 of incubation, and the embryos were examined on day 19. Number of embryonic death, developmental abnormalities and body weight of embryos were recorded on day 19. The body weight of embryos reduced significantly, the rate of embryo mortality increased, but teratogenic effect was not realised by the single treatment of lead acetate and cadmium sulphate. By the single administration of PYRINEX 48 EC the average body weight of embryos resulted in a significant decrease and the rate of embryo mortality and developmental anomalies were increased and it was found to be embryo toxic and teratogenic in the embryos. The increased embryo toxic effect can occur by the additive effect of the heavy elements (cadmium sulphate and lead acetate) and the PYRINEX 48 EC.