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Identification and Dissection of Diverse Mouse Adipose Depots
Published on: July 11, 2019
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Distinct Adipose Depots from Mice Differentially Respond to a High-Fat, High-Salt Diet
Vanessa C DeClercq1, Jennifer S Goldsby1, David N McMurray2
1Program in Integrative Nutrition and Complex Diseases.
The Journal of Nutrition
|May 6, 2016
Summary
High-fat diets induce inflammation and alter adipokine secretion in mice. High salt did not worsen these effects, but specific adipose depots responded differently to diet, impacting cellular function.
Area of Science:
- Metabolic research
- Obesity research
- Adipose tissue biology
Background:
- High-fat (HF) and high-sodium diets can promote inflammation.
- Limited data exists on how intra-abdominal adipose depots respond to combined high-salt, HF diets.
Purpose of the Study:
- To investigate the impact of high-fat (HF) feeding and high-salt addition on distinct adipose depots.
- To test if high salt exacerbates diet-induced changes in adipose tissue.
Main Methods:
- Male C57BL6 mice were fed control, HF, HF + high-salt, or low-fat + high-salt diets for 12 weeks.
- Serum and adipose tissue organ cultures were analyzed for adipokines.
- RNA sequencing and Ingenuity Pathway Analysis were used for functional analysis.
Main Results:
- HF diet induced inflammation and reduced adiponectin. High salt did not worsen these changes.
- Leptin and interleukin-6 release varied by depot; HF impaired adiponectin and resistin secretion.
- High-salt addition to HF diet did not further alter secretion in cultured adipose tissue.
Conclusions:
- Distinct adipose depots play a role in obesity development.
- Specific depots are crucial for studying therapeutic interventions targeting adipose tissue function.
