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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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Published on: September 21, 2017

Triplex-forming peptide nucleic acid modified with 2-aminopyridine as a new tool for detection of A-to-I editing

Chiara Annoni¹, Tamaki Endoh, Dziyana Hnedzko

¹Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan. sugimoto@konan-u.ac.jp.

Chemical Communications (Cambridge, England)

|May 10, 2016

Abstract:

RNA editing from adenosine to inosine (A-to-I editing) is one of the mechanisms that regulate and diversify the transcriptome. Here, a triplex-forming peptide nucleic acid (PNA) modified with a 2-aminopyridine nucleobase was applied for the recognition of the A-to-I editing event in double-stranded RNAs. The triplex-forming PNA enabled sequence-specific detection of single nucleobase editing at sub-nanomolar concentration.

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