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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
Published on: October 25, 2018
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Direct and reversible immobilization and microcontact printing of functional proteins on glass using a genetically
Brandon L Coyle1, François Baneyx1
1Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA, USA.
Chemical Communications (Cambridge, England)
|May 10, 2016
Abstract:
Fusion of disulfide-constrained or linear versions of the Car9 dodecapeptide to model fluorescent proteins support their on-contact and oriented immobilization onto unmodified glass. Bound proteins can be released and the surface regenerated by incubation with l-lysine. This noncovalent chemistry enables rapid and reversibe microcontact printing of tagged proteins and speeds up the production of bicontinuous protein patterns.
