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A study of separation selectivity using embedded ester-bonded stationary phases for liquid chromatography
Szymon Bocian1, Katarzyna Krzemińska1, Bogusław Buszewski1
1Chair of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland. bocian@chem.umk.pl.
The Analyst
|May 13, 2016
Summary
New stationary phases for liquid chromatography were synthesized using diol-modified silica. These novel materials effectively separate diverse compounds in reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILC), even with water as the mobile phase.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Development of novel stationary phases is crucial for advancing liquid chromatography techniques.
- Existing stationary phases may have limitations in separating specific compound classes or require organic solvents.
Purpose of the Study:
- To synthesize and characterize new stationary bonded phases for liquid chromatography.
- To evaluate the separation capabilities of these phases in both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILC) modes.
Main Methods:
- Synthesis of stationary phases by bonding various functional groups (alkyl, phenyl, cholesterol) to diol-modified silica via ester linkages.
- Structural confirmation using elemental analysis, FTIR, and (13)C CP/MAS NMR.
- Chromatographic evaluation under RP and HILC conditions.
Main Results:
- Successful synthesis of stationary phases with diverse functionalities.
- Demonstrated ability to separate various compound classes in both RP and HILC.
- Effective separation performance observed even when using pure water as the mobile phase.
Conclusions:
- The synthesized Diol-Ester stationary phases offer versatile separation capabilities for liquid chromatography.
- These novel phases show promise for applications requiring simplified mobile phases, including pure water.
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