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Related Experiment Video

Updated: Mar 21, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.

Daniel J Royston¹, Steven Clasper², David G Jackson³

¹MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2016

Summary

This summary is machine-generated.

Marker proteins are crucial for lymphatic research, enabling identification and isolation of lymphatic endothelial cells. This chapter details immunohistochemical analysis of lymphangiogenesis using these markers.

Keywords:

Chalkley grid Immunofluorescence LYVE-1 Lymphangiogenesis Lymphatic Peroxidase Podoplanin

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Last Updated: Mar 21, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

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Published on: February 9, 2020

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

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Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

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Published on: August 9, 2019

Area of Science:

Biomedical research
Cell biology
Pathology

Background:

Marker proteins are essential tools in lymphatic research.
They facilitate identification and isolation of lymphatic endothelial cells.
These markers aid in tumor pathology and basic research.

Purpose of the Study:

To focus on the use of marker proteins in immunohistochemical analysis of lymphangiogenesis.
To discuss current protocols and newer techniques.
To address associated problems in lymphatic research.

Main Methods:

Immunohistochemical analysis of lymphangiogenesis.
Utilizing marker proteins in frozen and paraffin-embedded tissue sections.
Employing biotin tyramide amplification techniques.

Main Results:

Marker proteins enable detailed analysis of lymphangiogenesis.
Established protocols and newer amplification methods are discussed.
Potential problems and limitations of current techniques are highlighted.

Conclusions:

Marker proteins significantly advance lymphatic research and diagnostics.
Immunohistochemistry is a key method for studying lymphangiogenesis.
Further refinement of protocols is needed for optimal results.