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Updated: Mar 21, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

A pragmatic guide to multiphoton microscope design.

Michael D Young¹, Jeffrey J Field², Kraig E Sheetz³

¹Center for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA.

Advances in Optics and Photonics

|May 17, 2016

Summary

This summary is machine-generated.

This guide provides a comprehensive resource for building and evaluating multiphoton microscopes. It covers essential components and design considerations for researchers across various scientific fields.

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Area of Science:

Biophysics
Optical Engineering
Microscopy

Background:

Multiphoton microscopy is a vital technique for visualizing microscopic structures and functions.
Its application spans diverse scientific disciplines, necessitating accessible design and construction resources.

Purpose of the Study:

To present a comprehensive guide for constructing and evaluating multiphoton microscopes.
To make advanced microscopy accessible to a wider range of scientific researchers.

Main Methods:

Detailed discussion on source selection and optical dispersion management.
Guidance on image relay systems, scan optics, and objective lens selection.
Explanation of light collection theory, photon-counting detection, and image rendering.

Main Results:

A practical, illustrated guide for building an example multiphoton microscope.
A framework for performance evaluation of custom-built systems.

Conclusions:

Democratizes access to multiphoton microscopy technology.
Empowers researchers to build and optimize their own advanced imaging systems.