Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

356
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
356
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.1K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

571
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
571

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scattering-enabled epi-quantitative phase imaging reveals subcellular detail in organoids and deep mouse brains.

bioRxiv : the preprint server for biology·2026
Same author

Tracing chalcolithic population mobility using strontium isotopes and proteomics at Gumelnița site, Romania.

Scientific reports·2025
Same author

Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part II.

Journal of biomedical optics·2024
Same author

Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part I.

Journal of biomedical optics·2024
Same author

Roadmap on Label-Free Super-Resolution Imaging.

Laser & photonics reviews·2024
Same author

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos†.

Biology of reproduction·2024

Related Experiment Video

Updated: Sep 25, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Spatial light interference microscopy: principle and applications to biomedicine.

Xi Chen1, Mikhail E Kandel1, Gabriel Popescu1

  • 1Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Advances in Optics and Photonics
|May 2, 2022
PubMed
Summary

Spatial Light Interference Microscopy (SLIM) offers sensitive, speckle-free quantitative phase imaging. This review covers SLIM

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.4K

Related Experiment Videos

Last Updated: Sep 25, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.4K

Area of Science:

  • Optical microscopy
  • Quantitative phase imaging
  • Biomedical optics

Background:

  • Quantitative Phase Imaging (QPI) is crucial for label-free cell analysis.
  • Spatial Light Interference Microscopy (SLIM) is a highly sensitive QPI technique.
  • SLIM builds upon phase-contrast microscopy with white-light illumination.

Purpose of the Study:

  • To provide a comprehensive review of Spatial Light Interference Microscopy (SLIM).
  • To detail SLIM's theory, instrumentation, and applications in science and medicine.
  • To highlight advancements and future directions in SLIM technology.

Main Methods:

  • Review of image formation in QPI, scattering, and full-field methods.
  • Detailed explanation of SLIM imaging, including theory, instrumentation, and diffraction tomography.
  • Discussion of Zernike phase-contrast microscopy, phase retrieval, halo removal, and inverse problem solutions (white-light and Wolf phase tomography).

Main Results:

  • SLIM enables speckle-free phase reconstruction with sub-nanometer path-length stability.
  • In-house software facilitates high-throughput acquisition, whole slide scanning, and mosaic tile registration.
  • SLIM demonstrates broad applicability in studying cell dynamics, growth, migration, and mass transport.

Conclusions:

  • SLIM is a powerful tool for quantitative phase imaging with diverse applications in basic science and clinical studies.
  • SLIM aids in cancer research, reproductive technology, and blood testing.
  • The integration of SLIM with artificial intelligence presents new avenues for cell biology and pathology research.