Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing Italian biophysics: insights from the 27th SIBPA congress.

European biophysics journal : EBJ·2026
Same author

Antisecretory Factor in rat cerebellar granule cells augments inhibitory current via GABA<sub>A</sub> receptors: a mechanism for vertigo reduction in Menière's disease?

European biophysics journal : EBJ·2026
Same author

Wavefront estimation through structured detection in laser scanning microscopy.

Biomedical optics express·2026
Same author

Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.

Biomolecules·2026
Same author

Visualizing intraorganellar ultrastructures, dynamics, and interactions with open-access background-free Lock-in-SIM.

Nature communications·2025
Same author

Darkfield Mueller matrix microscope allows the observation of single cells with high contrast.

Optics letters·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Mar 21, 2026

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
07:42

Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

Published on: July 20, 2022

3.5K

Pushing phase and amplitude sensitivity limits in interferometric microscopy.

Poorya Hosseini, Renjie Zhou, Yang-Hyo Kim

    Optics Letters
    |May 19, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Photon shot noise limits interferometric microscopy. Using a high-capacity camera significantly reduces this noise, achieving picometer-level optical path length stability in advanced interferometers.

    More Related Videos

    Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
    10:25

    Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

    Published on: December 20, 2016

    17.6K
    Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
    13:04

    Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

    Published on: January 18, 2022

    5.0K

    Related Experiment Videos

    Last Updated: Mar 21, 2026

    Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains
    07:42

    Optimizing Magnetic Force Microscopy Resolution and Sensitivity to Visualize Nanoscale Magnetic Domains

    Published on: July 20, 2022

    3.5K
    Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
    10:25

    Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

    Published on: December 20, 2016

    17.6K
    Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
    13:04

    Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

    Published on: January 18, 2022

    5.0K

    Area of Science:

    • Optical Physics
    • Microscopy Techniques
    • Metrology

    Background:

    • Interferometric microscopy relies on precise amplitude and phase measurements.
    • Measurement sensitivity is affected by instrument design and environmental factors.

    Purpose of the Study:

    • Identify the primary limiting factor in interferometric microscopy measurements.
    • Demonstrate a method to enhance measurement stability and precision.

    Main Methods:

    • Developed a theoretical framework to analyze noise sources.
    • Conducted experimental validation of the theoretical model.
    • Utilized a state-of-the-art camera with high full well capacity.

    Main Results:

    • Photon shot noise was identified as the dominant limiting factor.
    • The high-capacity camera significantly reduced shot noise.
    • Achieved optical path length stability down to a few picometers.

    Conclusions:

    • Photon shot noise is a critical bottleneck in interferometric microscopy.
    • Advanced camera technology can overcome shot noise limitations.
    • Picometer-level stability is achievable in near-common-path interferometers.