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Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation
Mahmood H Akhtar1, Tanveer A Mir1, N G Gurudatt1
1Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea.
Biosensors & Bioelectronics
|May 23, 2016
Summary
A novel sensor detects β-nicotinamide adenine dinucleotide (NADH) using ethylenediaminetetraacetic acid (EDTA) on graphene oxide and polyethylenimine. This sensor offers highly sensitive NADH detection for potential biomedical applications.
Area of Science:
- Electrochemistry
- Materials Science
- Biomedical Sensing
Background:
- β-nicotinamide adenine dinucleotide (NADH) is a crucial biomarker in biological systems.
- Sensitive and reliable detection of NADH is essential for various applications, including disease diagnosis.
- Existing NADH detection methods often face limitations in sensitivity, selectivity, or operational potential.
Purpose of the Study:
- To develop a robust amperometric sensor for highly sensitive β-nicotinamide adenine dinucleotide (NADH) detection.
- To investigate the electrochemical properties and complex formation of NADH with ethylenediaminetetraacetic acid (EDTA) on a modified graphene oxide/polyethylenimine platform.
- To evaluate the sensor's performance and reliability for detecting NADH in biological samples.
Main Methods:
- Fabrication of a sensor probe (GCE/AGO/PEI-EDTA) by immobilizing EDTA on activated graphene oxide (AGO) and polyethylenimine (PEI) layers.
- Electrochemical characterization using amperometry to assess NADH oxidation.
- Surface analysis techniques including quartz crystal microbalance (QCM) and X-ray photoelectron spectroscopy (XPS) to study material interactions.
- Evaluation of sensor performance, including dynamic range and detection limit, and testing in biological samples (tumorigenic lung epithelial cells).
Main Results:
- The developed sensor (GCE/AGO/PEI-EDTA) exhibited catalytic activity towards NADH oxidation at a low potential.
- Surface characterization confirmed the interaction between AGO, PEI, and EDTA, enhancing NADH interaction.
- The sensor demonstrated a wide dynamic range (0.05μM to 500μM) and a low detection limit (20.0±1.1nM).
- Successful detection of NADH in tumorigenic lung epithelial cells using the standard addition method validated its reliability.
Conclusions:
- The organic complex formation between EDTA and NADH on the AGO/PEI layer enables sensitive amperometric detection.
- The developed sensor shows excellent performance characteristics and potential for real-world biomedical applications.
- This approach offers a promising strategy for developing advanced electrochemical biosensors.
