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ELISA for human pancreatic elastase 1
A Sziegoleit1, H Knäpler, B Peters
1Institute of Medical Microbiology, University of Giessen, FRG.
Clinical Biochemistry
|April 1, 1989
Abstract:
A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for the RIA. In addition, the ELISA could be valid as a screening test for cystic fibrosis. Because ELISA reagents are relatively stable, the assay is always ready to use and can be performed without special equipment.