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Updated: Mar 20, 2026

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
Published on: November 18, 2022
[Alteration of intestinal permeability: the missing link between gut microbiota modifications and inflammation in
Laurent Genser1, Christine Poitou2, Édith Brot-Laroche3
1Institut de cardiométabolisme et nutrition, ICAN, hôpital Pitié-Salpêtrière, F-75013, Paris, France - Sorbonne universités, université Paris 06, UMR-S 1166, Nutriomics Team, F-75013 Paris, France - Inserm, UMRS 1166, Nutriomics, F-75013 5Paris, France.
Abstract:
The increasing incidence of obesity and associated metabolic complications is a worldwide public health issue. The role of the gut in the pathophysiology of obesity, with an important part for microbiota, is becoming obvious. In rodent models of diet-induced obesity, the modifications of gut microbiota are associated with an alteration of the intestinal permeability increasing the passage of food or bacterial antigens, which contribute to low-grade inflammation and insulin resistance. In human obesity, intestinal permeability modification, and its role in the crosstalk between gut microbiota changes and inflammation at systemic and tissular levels, are still poorly documented. Hence, further characterization of the triggering mechanisms of such inflammatory responses in obese subjects could enable the development of personalized intervention strategies that will help to reduce the risk of obesity-associated diseases.
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