Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SynaptoTagMe, a toolkit for in vivo mapping and modulating neurotransmission at single-cell resolution.

eLife·2026
Same author

Distinct Brain Systems Support Afferent and Efferent Autonomic Activity.

bioRxiv : the preprint server for biology·2026
Same author

A modular framework for automated segmentation and analysis of AFM imaging of chromatin organization.

Nucleic acids research·2026
Same author

Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Author Correction: Shared receptors in axon guidance: SAX-3/Robo signals via UNC-34/Enabled and a Netrin-independent UNC-40/DCC function.

Nature neuroscience·2026
Same author

Combinatorial histone modifications direct ATP-dependent chromatin remodeling by NURF to promoter-proximal nucleosomes.

Nucleic acids research·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Mar 20, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K

Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging.

Sara Abrahamsson1, Rob Ilic2, Jan Wisniewski3

  • 1HHMI and Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA; Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Biomedical Optics Express
|May 28, 2016
PubMed
Summary
This summary is machine-generated.

We developed advanced diffractive optics for multifocus microscopy (MFM) to improve 3D imaging speed and brightness. These innovations enable faster, clearer biological sample visualization with reduced photodamage.

Keywords:
(050.1970) Diffractive optics(110.4190) Multiple imaging(180.2520) Fluorescence microscopy(180.6900) Three-dimensional microscopy(260.5430) Polarization

More Related Videos

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

14.1K
Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.7K

Related Experiment Videos

Last Updated: Mar 20, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
11:24

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

14.1K
Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States
06:25

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

1.7K

Area of Science:

  • Optical microscopy
  • Nanofabrication
  • Biophysics

Background:

  • Multifocus microscopy (MFM) enables rapid 3D imaging of biological samples.
  • Optimizing light efficiency is crucial for live imaging, balancing speed and minimizing photodamage.

Purpose of the Study:

  • To design and fabricate highly efficient diffractive multifocus gratings (MFGs) for MFM.
  • To implement a precise color MFM system for enhanced fluorophore imaging.
  • To improve volumetric time-lapse imaging capabilities.

Main Methods:

  • Developed novel pattern designs for diffractive optics.
  • Utilized nanofabrication techniques for MFG production.
  • Implemented a multi-phase MFG for neuronal imaging.

Main Results:

  • Achieved theoretical efficiencies of ≈90% and manufactured efficiencies >80% for MFGs.
  • Demonstrated faster and brighter volumetric imaging.
  • Successfully applied the technology to 3D functional neuronal imaging in C. elegans.

Conclusions:

  • Advanced MFG designs significantly enhance MFM performance.
  • The developed methods enable efficient, high-resolution 3D live imaging.
  • This work provides tools and insights for optimizing MFM in biological research.