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Updated: Mar 20, 2026
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
Published on: March 5, 2017
Genetic Modification of Human Pancreatic Progenitor Cells Through Modified mRNA
Song Lu1,2, Christie C Chow2, Junwei Zhou3
1Department of Chemical Pathology, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.
Abstract:
In this chapter, we describe a highly efficient genetic modification strategy for human pancreatic progenitor cells using modified mRNA-encoding GFP and Neurogenin-3. The properties of modified mRNA offer an invaluable platform to drive protein expression, which has broad applicability in pathway regulation, directed differentiation, and lineage specification. This approach can also be used to regulate expression of other pivotal transcription factors during pancreas development and might have potential therapeutic values in regenerative medicine.
