[Highly Frequent Regeneration System Establishment of Lycium ruthenicum]

To establish a rapid regeneration system of Lycium ruthenicum in order to produce large-scale commodity seedlings.

Cotyledon, hypocotyl and radicle of Lycium ruthenicum seedlings were used as explants to produce plantlets by depending on MS basal medium mixed with the different kinds and different concentration of hormones.

The desirable hormone composition for the inducement was MS + 6-BA 1.0 mg/L + NAA 0.5 mg/L, and the callus induction rate was as high as 100%, the optimum medium for the buds sprouting and regeneration was that of MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L, with a propagation coefficient of 7.73, and the suitable medium for the roots induction was 1/2MS + IBA 1.0 mg/L, 90% of the regenerated plantlets transferred to an open field survive and grow well.

The callus can be induced by cotyledon, hypocotyls and radicle of Lycium ruthenicum seedlings, and hypocotyls is the best explants for callus induced. The formulations suitable for callus induction, differentiation, bud proliferation and rooting are screened out to establish the rapid regeneration system for Lycium ruthenicum.