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[UPLC Fingerprints of Euphorbiae Ebracteolatae Radix and Its Vinegar-Processed Slices]
Objective:
To compare the qualities of Euphorbiae Ebracteolatae Radix from different original plants and its vinegar-processed slices.
Methods:
UPLC fingerprints of Euphoria ebracteolata, Euphoria fischeriana and vinegar-processed slices of Euphoria ebracteolata were established on a Waters ODS column (100 mm x 2.1 mm, 1.8 μm), using jolkinolide B as the reference substance. A mixture of acetonitrile and water was used as the mobile phase in a gradient elution mode, with the flow rate of 0.4 mL/min, the column temperature at 30 degrees C and the UV detector at 210 nm. The UPLC analysis time was no more than 20 min.
Results:
The qualities of Euphoria ebracteolata and its vinegar-processed slices were stable with similarities over 0.90, respectively. However, the fingerprints of the slices and their original crude drugs exhibited obvious difference on both number and the relative peak area of the common peaks. Meanwhile the similarities of the fingerprints of Euphoria fischeriana and Euphoria ebracteolata were less than 0.72, indicating significant difference of these two plants.
Conclusion:
The developed method is available for quality control of Euphorbiae Ebracteolatae Radix and mechanism exploration of its vinegar-processed slices.
