Related Experiment Video
Updated: Mar 19, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Automated Glycan Assembly of Complex Oligosaccharides Related to Blood Group Determinants
Heung Sik Hahm1, Chien-Fu Liang1, Chian-Hui Lai1
1Department of Biomolecular Systems, Max Planck Institute for Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.
Abstract:
Lactotetraosyl (Lc4) and neo-lactotetraosyl (nLc4) are backbones that are common to many glycans. Using automated glycan assembly, these common core structures were constructed and elaborated to access synthetically challenging glycans of biological relevance. The incorporation of α-fucoses is demonstrated for H-type I and II; α(1,3)-galactose epitopes were prepared, and the pentasaccharide HNK-1 required incorporation of a 3-O-sulfate. In addition to preparing the target structures, essential insights were gained regarding the relationships of glycosylating agents and nucleophiles as well as the linker stability.
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Glycosylation
Glycosylation occurs in...
Proteoglycans

