Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Toward autonomous robotic-assisted and microrobotic surgery.

Science advances·2026

Same author

Fluorescence-Guided Surgery of Rhabdomyosarcoma Using a B7-H3 Targeted Antibody-Fluorophore Conjugate.

Molecular pharmaceutics·2026

Same author

Phantom Calibration's Impact on Measuring Radiodensity Changes: Implications for Evaluating Osteosarcoma Response.

Academic radiology·2026

Same author

In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.

bioRxiv : the preprint server for biology·2026

Same author

Implementation of a Prospective, Multi-Center Clinical Study of Dynamic Contrast Fluorescence Imaging to Diagnose Necrotizing Soft-Tissue Infection - Challenges and Lessons Learned.

Proceedings of SPIE--the International Society for Optical Engineering·2026

Same author

Tensile expansion microscopy applies mechanical force to super-resolve fixed and image live cellular samples.

bioRxiv : the preprint server for biology·2026

Same journal

Application of ephrin-B2 loaded glycol chitosan-silk fibroin hydrogel in the treatment of diabetic refractory wounds.

Scientific reports·2026

Same journal

International expert Delphi consensus on thromboprophylaxis in metabolic and bariatric surgery.

Scientific reports·2026

Same journal

Assessing the cross-region knowledge transfer capability of selected deep learning building vectorization methods in the context of available training datasets.

Scientific reports·2026

Same journal

Feasibility and preliminary effects of outdoor versus indoor cognitive-motor therapy in women with Alzheimer's disease: A randomized single-blind pilot study.

Scientific reports·2026

Same journal

Hallmarks of social action in the vocal turn-taking of wild common marmosets (Callithrix jacchus).

Scientific reports·2026

Same journal

Role and mechanism of AOPPs-induced NOX4-mediated ferroptosis in intervertebral disc degeneration.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Programmable DNA Nanosystem for Molecular Interrogation.

Divita Mathur^1,2,3,4, Eric R Henderson^1,2

¹Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.

Scientific Reports

|June 9, 2016

Summary

This summary is machine-generated.

This study introduces a novel DNA nanosystem capable of detecting molecular interactions. The system utilizes DNA-based forces to interrogate molecular binding at a specific interface, successfully detecting a viral genome mimic.

More Related Videos

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Related Experiment Videos

Last Updated: Mar 19, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Area of Science:

Nanotechnology
Molecular Biology
Biochemistry

Background:

Developing sophisticated tools for molecular interaction analysis is crucial in nanotechnology and molecular biology.
DNA nanotechnology offers a versatile platform for constructing nanoscale devices with precise control.

Purpose of the Study:

To present a self-assembling DNA-based nanosystem designed for interrogating molecular interactions.
To demonstrate the system's capability in detecting molecular adhesion and a specific nucleic acid mimic.

Main Methods:

Construction of a DNA nanosystem featuring a dumbbell-shaped frame, a central core, and a mobile ring.
Utilizing DNA strand transitions (single-stranded to double-stranded) to generate forces controlling ring motion.
Employing the ring-frame interface to detect molecular interactions, including base stacking adhesion.

Main Results:

The DNA nanosystem successfully self-assembled as designed.
The system demonstrated the ability to interrogate molecular interactions by controlling ring movement against adhesive forces.
Proof-of-principle experiments successfully detected base stacking adhesion and a soluble nucleic acid viral genome mimic.

Conclusions:

The developed DNA nanosystem provides a novel mechanism for molecular detection and interrogation at the nanoscale.
This platform shows promise for analyzing molecular binding events and detecting specific nucleic acid targets.