[A new method of determining antibody heterogenicity (among them, monoclonal) by affinity]

In this assay, cellulose antigenic immunosorbent was used as solid phase and preparations of polyclonal and monoclonal bivalent antibodies as antibodies. The new method is based on the assumption that definite relationship exists between some of free molecules and some of bound active centers of antibodies in the process of the interaction of antibodies and antigenic immunosorbent. Groups of antibodies with different affinity were detected in hyperimmune horse serum, in immune mouse serum and in the monoclonal preparation, which, on the one hand, confirms our earlier data and, on the other hand, demonstrates the possibility of using the new method for determination of antibody affinity.