Two new phenols from Lysimachia patungensis

Area of Science:

• Phytochemistry
• Natural Products Chemistry
• Pharmacology

Background:

• The genus Lysimachia is known for its diverse phytochemical constituents.
• Phenolic compounds are recognized for their potential health benefits, including antioxidant properties.
• Lysimachia patungensis Hand.-Mazz. is a plant species with limited phytochemical investigation.

Purpose of the Study:

• To isolate and characterize phenolic compounds from Lysimachia patungensis.
• To evaluate the potential protective effects of isolated compounds against oxidative stress in human retinal endothelial cells (HRECs).

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation using spectroscopic methods (ESI-MS, HR-TOF-MS, UV, IR, NMR).
• In vitro bioassay using hydrogen peroxide-induced damage in HRECs.

Main Results:

• Two new phenolic compounds, methyl 3-(2-O-β-d-glucopyranosyl-3-hydroxy-5-methoxyphenyl) propionate (1) and myricetin-3,3',5'-tri-O-α-l-rhamnopyranoside (2), were identified.
• Six known phenolic compounds (3-8) were isolated; compounds 3-8 were reported from the Lysimachia genus for the first time.
• Compounds 3 and 7 demonstrated significant protective effects against H2O2-induced damage in HRECs at 10 μM.
• Compound 1 exhibited moderate activity in protecting HRECs from damage at 100 μM.

Conclusions:

• The study successfully isolated and identified novel and known phenolic compounds from Lysimachia patungensis.
• Compounds 3, 7, and 1 show potential as therapeutic agents for retinal protection against oxidative stress.
• Further research is warranted to explore the mechanisms of action and therapeutic potential of these compounds.