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Thin-layer chromatography of free porphyrins for diagnosis of porphyria
1Department of Chemical Pathology, St. James's University Hospital, Leeds, U.K.
Clinical Chemistry
|June 1, 1989
Abstract:
In this simple thin-layer chromatographic (TLC) technique for evaluating porphyrin excretion, porphyrins are extracted from urine or feces, then separated on silica-gel TLC plates. The distinct porphyrin bands are observed by viewing the plates under long-wave fluorescent light. Positive screening tests can readily be confirmed or rejected, and a more comprehensive investigation confidently undertaken.

