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Updated: Mar 19, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
Application of Fluorescence Polarization in HTS Assays
1Immunology, Inflammation and Infectious Diseases Discovery and Translational Area Roche Pharma Research & Early Development, Roche Innovation Center Shanghai, Roche R&D Center (China) Ltd, 720 Cai Lun Road, Bldg 5, Pudong, Shanghai, 201203, China. xinyi.huang@roche.com.
Abstract:
Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

