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Updated: Mar 19, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Exploration of upstream and downstream process for microwave assisted sustainable biodiesel production from
Amit Kumar Sharma1, Pradeepta Kumar Sahoo2, Shailey Singhal3
1Biofuel Research Laboratory, University of Petroleum and Energy Studies, Bidholi, Dehradun 248007, India.
Abstract:
The present study explores the integrated approach for the sustainable production of biodiesel from Chlorella vulgaris microalgae. The microalgae were cultivated in 10m(2) open raceway pond at semi-continuous mode with optimum volumetric and areal production of 28.105kg/L/y and 71.51t/h/y, respectively. Alum was used as flocculent for harvesting the microalgae and optimized at different pH. Lipid was extracted using chloroform: methanol (2:1) and having 12.39% of FFA. Effect of various reaction conditions such as effect of catalyst, methanol:lipid ratio, reaction temperature and time on biodiesel yields were studied under microwave irradiation; and 84.01% of biodiesel yield was obtained under optimized reaction conditions. A comparison was also made between the biodiesel productions under conventional heating and microwave irradiation. The synthesized biodiesel was characterized by (1)H NMR, (13)C NMR, FTIR and GC; however, fuel properties of biodiesel were also studied using specified test methods as per ASTM and EN standards.
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