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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Mar 19, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation.

Kui Chen1,2, Mingyi Zhang1, Ya-Nan Chang1

  • 1CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing, 100049, China.

Nanoscale Research Letters
|June 22, 2016
PubMed
Summary

Researchers developed a gold nanoparticle (GNP) probe for semi-quantitative analysis of methylated tumor suppressor genes. This cost-effective method aids in diagnosing DNA methylation-related diseases.

Keywords:
DNA methylationGold nanoparticles probeSemi-quantitative assaySurface plasmon resonanceVisual detection

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Surface plasmon resonance (SPR) enables gold nanoparticles (GNPs) to visualize biomolecules.
  • DNA methylation is crucial in tumor suppressor gene regulation and disease development.
  • Accurate detection of gene methylation is vital for disease diagnosis.

Purpose of the Study:

  • To design and construct a GNP-based probe for semi-quantitative analysis of methylated tumor suppressor genes in cultured cells.
  • To develop a simple, cost-effective method for diagnosing DNA methylation-related diseases.

Main Methods:

  • Gold nanoparticles (GNPs) were coated with single-stranded DNA (ssDNA) via Au-S bonds.
  • The ssDNA incorporated a regulatory domain and a functional domain for specific pairing with methylated p16 sequences (Met-p16).
  • Color changes in aggregating GNPs in NaCl solution indicated Met-p16 presence, enabling semi-quantification.

Main Results:

  • The GNP probe successfully detected and semi-quantified p16 gene methylation in HCT116 cells.
  • Methylation of E-cadherin, p15, and p16 genes was detected in Caco2, HepG2, and HCT116 cell lines using specific probes.
  • The method demonstrated a clear color change in response to target methylated sequences.

Conclusions:

  • The developed GNP probe offers a simple and cost-effective approach for detecting DNA methylation.
  • This method shows potential for the diagnosis of DNA methylation-related diseases.
  • The probe design is adaptable for detecting various methylated gene sequences.