Related Experiment Videos
A novel device to create consistent deep dermal burns in a porcine model
Seema Menon1, Queenie Chan2, Monique Bertinetti2
1Department of Paediatric Surgery, The Children's Hospital Burns Research Institute and Burns Unit, The Children's Hospital at WestmeadNew South Wales, Australia; Sydney Medical School, The University of SydneyNew South Wales, Australia.
International Journal of Burns and Trauma
|June 24, 2016
Summary
A novel device effectively creates consistent deep partial thickness burns in pigs. This tool aids research into hypertrophic scarring development using a reliable porcine burn model.
Area of Science:
- Biomedical Engineering
- Dermatology
- Veterinary Medicine
Background:
- Developing reliable animal models is crucial for studying burn wound healing and scar formation.
- Current methods for inducing burns in animal models may lack consistency and reproducibility.
- A standardized burn model is needed to investigate the pathogenesis of hypertrophic scarring.
Purpose of the Study:
- To evaluate a novel thermostatically controlled device for creating consistent deep partial thickness burns.
- To assess the reproducibility of burn depth and characteristics in a porcine model.
- To establish a reliable preclinical model for hypertrophic scar research.
Main Methods:
- A custom-designed, heated aluminum disc device (92°C) was used to create contact burns.
- Burns were induced on the flanks of two pigs with varying contact durations (5, 10, 15, 20 seconds).
- Histological analysis of burn depth was performed at 10 minutes (day 0) and 8 days post-burn.
Main Results:
- Histology at day 0 showed superficial to mid-dermal burns with minimal edema and necrosis.
- Histology at day 8 revealed mid to deep dermal edema and necrosis in all tested areas.
- A 20-second contact duration consistently produced deep dermal burns.
Conclusions:
- The novel heated disc device reliably generates reproducible deep partial thickness burns in a porcine model.
- The device's consistency supports its utility in preclinical burn research.
- This validated porcine burn model can be utilized for investigating hypertrophic scarring mechanisms.