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Droplet-based glucosamine sensor using gold nanoparticles and polyaniline-modified electrode
Akkapol Suea-Ngam1, Poomrat Rattanarat2, Kanet Wongravee3
1Chromatography and Separation Research Unit (ChSRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Electrochemistry and Optical Spectroscopy Research Unit (EOSRU), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
A new droplet-based electrochemical sensor using gold nanoparticles and polyaniline-modified electrodes offers high-throughput, precise D-glucosamine detection. This method prevents analyte adsorption, ensuring accurate measurements in supplements.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Analytical Chemistry
Background:
- Direct measurement of D-glucosamine is crucial for quality control in supplements.
- Existing methods may suffer from analyte adsorption and low throughput.
- Development of sensitive and efficient electrochemical sensors is needed.
Purpose of the Study:
- To develop a droplet-based electrochemical sensor for direct D-glucosamine measurement.
- To optimize electrode fabrication using Central Composition Design (CCD).
- To achieve high-throughput and accurate analysis of D-glucosamine.
Main Methods:
- Fabrication of carbon paste electrodes (CPEs) modified with gold nanoparticles (AuNPs) and polyaniline (PANI).
- Optimization of AuNPs and PANI concentrations using CCD.
- Integration with a droplet microfluidic system for high-throughput analysis.
- Electrochemical detection of D-glucosamine.
Main Results:
- Optimized AuNPs and PANI concentrations were 300 and 3000mgL(-1).
- The sensor achieved a sample throughput of at least 60 samples/hour.
- Linearity was observed in the 0.5-5mM range with a sensitivity of 7.42×10(-3)Amol(-1)Lcm(-2).
- Limits of detection and quantitation were 0.45mM and 1.45mM, respectively.
- High precision (RSD < 3%) and accuracy (error < 3%) were demonstrated in supplement analysis.
Conclusions:
- The developed droplet-based electrochemical sensor provides a sensitive, precise, and high-throughput method for D-glucosamine determination.
- Compartmentalization in droplets effectively prevents analyte adsorption.
- The sensor shows excellent performance and agreement with conventional methods, suitable for analyzing real samples.
