Polycyclic Aromatic Hydrocarbons: Part II, Urine Markers

There has been increasing demand for simple, rapid, highly sensitive, inexpensive yet reliable method for detecting predisposition to cancer. Human biomonitoring of exposure to the largest class of chemical carcinogen, polycyclic aromatic hydrocarbons (PAHs) that are rapidly transformed into detectable metabolites (eg, 1-hydroxypyrene), can serve as strong pointers to early detection of predisposition to cancer. Given that any exposure to PAH is assumed to pose a certain risk of cancer, several biomarkers have been employed in biomonitoring these ninth most threatening ranked compounds. They include metabolites in urine, urinary thioethers, urinary mutagenicity, genetoxic end points in lymphocytes, hemoglobin adducts of benzo(a)pyrene, PAH-protein adducts, and PAH-DNA adducts among others. In this chapter, the main focus will be on the urine metabolites since urine samples are easily collected and there is a robust analytical instrument for the determination of their metabolites.