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[Clinical studies on urinary tissue polypeptide antigen (TPA): problems and improvement in determination]
M Yoshida1, S Morimoto, Y Uekado
1Department of Urology, Wakayama Medical College.
Hinyokika Kiyo. Acta Urologica Japonica
|February 1, 1989
Summary
Urinary TPA measurement is unreliable due to sample instability. Treating urine with Tween 40 stabilizes tissue polypeptide antigen (TPA) and improves assay accuracy, preventing false positives.
Area of Science:
- Biochemistry
- Clinical Chemistry
Context:
- Urinary tissue polypeptide antigen (TPA) measurement is crucial for disease monitoring.
- Existing TPA assay methods face challenges with reliability and false positive rates.
Purpose:
- To investigate the reliability of urinary TPA measurement using the Prolifigen TPA kit.
- To develop and test an improved method for stabilizing urinary TPA.
Summary:
- Filtration of urine samples did not improve TPA reliability due to TPA instability in untreated samples.
- Tween 40 treatment stabilized TPA, yielding accurate dilution and recovery test results.
- Tween 40 prevented TPA value decrease during 24-hour storage at room temperature.
Impact:
- The findings highlight the instability of urinary TPA and the inadequacy of simple filtration for purification.
- Tween 40 treatment offers a viable method to enhance the stability and reliability of urinary TPA measurements.
- This improved method has the potential to reduce false positives and improve diagnostic accuracy in clinical settings.