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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Modern Molecular Taxonomy01:29

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Updated: Mar 18, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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HLA Genotyping using Next Generation Sequencing.

C Lucan, Laura-Ancuta Pop, A Florian

    Romanian Journal of Internal Medicine = Revue Roumaine De Medecine Interne
    |June 29, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Pediatric brain tumors are a significant challenge, with stem cell transplantation offering a promising avenue. Next-generation sequencing for HLA typing is crucial for successful stem cell transplants in these young patients.

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    Area of Science:

    • Pediatric oncology
    • Neuro-oncology
    • Transplantation immunology

    Background:

    • Pediatric brain tumors represent the second most common childhood malignancy.
    • Despite advances in surgery, radiotherapy, and chemotherapy, curability remains poor for high-risk and recurrent cases.
    • Aggressive tumor infiltration and location make complete surgical removal difficult, leading to high treatment failure and recurrence rates.

    Purpose of the Study:

    • To review the role of next-generation sequencing (NGS) in human leukocyte antigen (HLA) typing.
    • To explore the application of NGS-based HLA typing in stem cell transplantation for pediatric central nervous system (CNS) malignancies.

    Main Methods:

    • Review of current literature on stem cell transplantation for pediatric CNS tumors.
    • Focus on the advancements and utility of next-generation sequencing (NGS) for human leukocyte antigen (HLA) typing.
    • Analysis of the impact of precise HLA matching on transplantation outcomes.

    Main Results:

    • Next-generation sequencing (NGS) provides high-resolution human leukocyte antigen (HLA) typing, essential for accurate donor-recipient matching.
    • Improved HLA typing accuracy can potentially enhance the efficacy and safety of stem cell transplantation.
    • Stem cell transplantation is emerging as a viable therapeutic strategy for challenging pediatric CNS malignancies.

    Conclusions:

    • Accurate human leukocyte antigen (HLA) typing via next-generation sequencing (NGS) is critical for optimizing stem cell transplantation outcomes in pediatric CNS malignancies.
    • This approach holds significant potential for improving the management of difficult-to-treat pediatric brain tumors.
    • Further research is warranted to fully integrate NGS-based HLA typing into clinical protocols for these patients.