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Updated: Mar 18, 2026
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
Published on: March 8, 2024
[Metabolites Identification for Alkaloids from Nelumbinis Plumula in Caco-2 Cells by LC/MS/MS]
Objective:
To identify the metabolites of norcoclaurine,liensinine, isoliensinine and neferine in Caco-2 cells by LC/ MS/MS.
Methods:
After Caco-2 cells were treated with norcoclaurine, liensinine, isoliensinine or neferine for 3, 6 and 12 h, samples were collected, purified and then analyzed by LC/MS/MS. The structures of the metabolites were elucidated by molecular masses, retention times, MS and MS/MS spectra comparing with those of the parent drug.
Results:
The procedure identified that the major metabolites of norcoclaurine were methylnorcoclaurine and norcoclaurine-glucuronide, the major metabolite of liensinine was demethyl-liensinine, the major metabolite of isoliensinine was demethyl-isoliensinine, the major metabolites of neferine were liensinine, isoliensinine and their further demethylation products.
Conclusion:
LC/MS/MS is simple, rapid and sensitive for the metabolites identification. Methylation, demethylation and glucuronidation are main metabolic pathways of alkaloids from Nelumbinis Plumula in Caco-2 cells.
