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Published on: October 17, 2018
Heterogeneous Circulating Angiogenic Cell Responses to Acute Maximal Exercise
Daniel D Shill1, Meagan P Marshburn, Hannah K Hempel
1Department of Kinesiology, University of Georgia, Athens, GA.
Insights
Acute maximal exercise significantly increases circulating angiogenic cells (CAC), including progenitor and non-progenitor types. These responses vary between sexes and may be influenced by menstrual cycle phase and contraceptive use.
Area of Science:
- Cardiovascular Physiology
- Exercise Immunology
- Endothelial Biology
Background:
- Circulating angiogenic cells (CAC) are crucial for angiogenesis and endothelial repair.
- CAC populations within peripheral blood mononuclear cells (PBMC) are modulated by exercise.
- Understanding exercise-induced CAC changes is vital for cardiovascular health research.
Purpose of the Study:
- To investigate the impact of acute maximal exercise on specific CAC subpopulations in healthy adults.
- To analyze changes in CD31, CD62E, CD14/CD31, CD34/VEGFR2, CD3/CD31, and CD3 PBMC.
- To explore sex-specific differences and potential influences of the menstrual cycle and contraceptive use on CAC responses.
Main Methods:
- Blood samples were collected pre- and post-maximal graded treadmill exercise.
- Flow cytometry was employed for the quantitative analysis of PBMC subpopulations.
- Specific cell markers (CD31, CD62E, CD14, CD34, VEGFR2, CD3) were assessed.
Main Results:
- Maximal exercise significantly increased lymphocytic CD31, monolymphocytic CD31, CD62E, CD14/CD31, and CD34/VEGFR2 PBMC.
- CD62E and CD14/CD31 PBMC increases were significantly higher in women.
- Menstrual cycle phase and contraceptive use showed potential modulatory effects on CAC levels.
Conclusions:
- Acute maximal exercise elicits a substantial CAC response involving diverse cell types.
- Sex-specific differences in CD62E and CD14/CD31 PBMC responses to exercise were observed.
- Menstrual cycle phase and contraceptive status may influence exercise-induced CAC mobilization.
Purpose:
Circulating angiogenic cells (CAC) comprise multiple subpopulations of exercise-inducible peripheral blood mononuclear cells (PBMC) that promote angiogenesis and maintain endothelial integrity. We examined the effect of acute maximal exercise on CD31, CD62E, CD14/CD31, CD34/VEGFR2, CD3/CD31, and CD3 PBMC in young, healthy adults.
Methods:
Blood samples were collected before and immediately after a graded treadmill exercise test for CAC analysis via flow cytometry.
Results:
Maximal exercised produced 40%, 29%, 33%, 14%, and 33% increases in lymphocytic CD31, monolymphocytic CD31, CD62E, CD14/CD31, and CD34/VEGFR2 PBMC, respectively (P < 0.05). CD3/CD31 and CD3 cells were not altered with exercise. CD62E and CD14/CD31 PBMC were selectively augmented in women by 54% and 20%, respectively (P < 0.05). Exploratory analyses indicated that maximal exercise induced greater increases in CD62E and CD14/CD31 PBMC among women in the luteal phase compared with those in the follicular phase (P < 0.05). Basal lymphocytic PBMC and postexercise lymphocytic and monolymphocytic CD31 PBMC were lower among contraceptive users than nonusers.
Conclusions:
Maximal exercise induces a robust CAC response encompassing both progenitor and nonprogenitor cell types, with these effects differing between men and women for CD62E and CD14/CD31 cell types and the potential influence of menstrual cycle phase and contraceptive use.
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