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Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
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Related Experiment Video

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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
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How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days.

I de Laak-de Vries1, A G Siebers1, L Burgers1

  • 1Department of Pathology 824, Radboud University Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.

Journal of Hematopathology
|July 12, 2016
PubMed
Summary
This summary is machine-generated.

Rapid microwave-enhanced processing of bone marrow biopsies (BMB) with USEDECALC and LOGOS J enables next-day reporting. This method preserves morphology and antigens while improving DNA quality for faster oncology diagnostics.

Keywords:
Bone marrow examinationDecalcification technique

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Area of Science:

  • Pathology
  • Oncology Diagnostics
  • Histotechnology

Background:

  • Traditional bone marrow biopsy (BMB) processing is time-consuming, involving lengthy fixation and decalcification.
  • The increasing demand for fast diagnostic tracks in oncology necessitates faster BMB processing methods.
  • Microwave-enhanced tissue processing offers a potential solution for rapid BMB preparation.

Purpose of the Study:

  • To evaluate a novel method for rapid BMB processing using microwave technology.
  • To assess the efficacy of a specific EDTA-based decalcification fluid (USEDECALC) combined with a microwave processor (LOGOS J).
  • To determine if this rapid processing impacts morphology, immunohistochemistry, or DNA quality.

Main Methods:

  • Bone marrow biopsies (BMB) were processed using a closed microwave-enhanced tissue processor (LOGOS J).
  • Overnight fixation, decalcification with an EDTA-based fluid (USEDECALC), and paraffin impregnation were performed.
  • Morphology, immunohistochemistry, and DNA quality were analyzed.

Main Results:

  • The microwave-enhanced processing allowed for overnight fixation, decalcification, and impregnation of BMB.
  • Histological morphology and immunohistochemistry results were preserved.
  • Improved DNA quality was observed in the processed BMB samples.
  • This method facilitates next-day reporting of BMB.

Conclusions:

  • Microwave-enhanced processing with USEDECALC and LOGOS J provides a rapid and effective method for BMB preparation.
  • This technique supports fast diagnostic tracks in oncology without compromising tissue quality or antigen integrity.
  • The improved DNA quality further enhances the utility of BMB for molecular analyses.