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Related Experiment Video

Updated: Mar 18, 2026

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

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Immunochromatographic Methods in Malaria Diagnosis.

M N Mishra¹, R N Misra²

¹Classified Specialist (Pathology and Immunopathology) 166 Military Hospital, C/o 56 APO.

Medical Journal, Armed Forces India

|July 14, 2016

Summary

The parasite lactate dehydrogenase (pLDH) test shows high accuracy for diagnosing malaria, especially Plasmodium vivax. While useful in the field, microscopy remains essential for laboratory confirmation.

Keywords:

Immunochromatography Malaria

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Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

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Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

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Published on: November 10, 2015

Area of Science:

Medical Diagnostics
Infectious Diseases
Parasitology

Background:

Malaria is a significant public health concern in India, contributing to substantial morbidity and mortality.
Accurate and rapid diagnostic tools are crucial for effective malaria control and treatment.

Purpose of the Study:

To evaluate the diagnostic utility of the parasite lactate dehydrogenase (pLDH) test for malaria.
To compare the pLDH test's performance against microscopy and histidine-rich protein-2 (HRP-2) antigen detection.

Main Methods:

A study involving 400 febrile patients, including 104 with malaria symptoms.
Comparison of pLDH test results with blood smear microscopy and an immunochromatographic test (ICT) for Plasmodium falciparum HRP-2.

Main Results:

The pLDH test demonstrated 100% sensitivity and specificity for Plasmodium vivax detection.
For Plasmodium falciparum, the pLDH test achieved 96% sensitivity and 100% specificity.

Conclusions:

Immunochromatographic tests (ICTs) are valuable for field diagnosis of Plasmodium falciparum malaria.
Microscopy of blood smears remains indispensable for accurate malaria diagnosis in laboratory settings.