Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lymphatic egress recycles tumor-experienced effector CD8 T cells to sustain immune surveillance.

bioRxiv : the preprint server for biology·2026

Same author

A tumor metabolism-angiogenesis-immune axis governs immunotherapy responses.

bioRxiv : the preprint server for biology·2026

Same author

Peripheral immune-inducer dendritic cells drive early-life allergic inflammation.

Nature·2026

Same author

Chronic Cholesterol Exposure Disrupts Macrophage Polarization and Cytokine Secretion in a 3D Microenvironment.

ACS omega·2026

Same author

The COSPAR Panel on Planetary Protection and the COSPAR Policy on Planetary Protection: an overview of governance and activities.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

Same author

Further updates to the Committee on Space Research Planetary Protection Policy for missions to Icy Worlds.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

Same journal

Potential role of myeloid bodies in protection against photo-oxidative damage of the retinal pigment epithelium.

Journal of cell science·2026

Same journal

From the FAZ to the TAC: Widespread cytoskeletal engagement of T. brucei KMP11.

Journal of cell science·2026

Same journal

Branched actin segregates endocytic cargo to control sorting and fission.

Journal of cell science·2026

Same journal

FAM122A inhibition of PP2A-B55 through a bipartite binding mechanism.

Journal of cell science·2026

Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026

Same journal

The force, form and function of the nucleus.

Journal of cell science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 17, 2026

Live Cell Imaging during Mechanical Stretch

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

Non-invasive single-cell biomechanical analysis using live-imaging datasets.

Yanthe E Pearson¹, Amanda W Lund², Alex W H Lin³

¹Department of Applied Mathematics and Sciences, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE.

Journal of Cell Science

|July 17, 2016

Summary

This summary is machine-generated.

This study presents a new computational tool for analyzing live cell dynamics from images. It measures cell shape, migration, stiffness, and viscosity, offering a non-invasive method for cell characterization.

Keywords:

Cell biomechanics Cell migration Live imaging Mechanobiology Mesenchymal stem cells T-lymphocyte cells

More Related Videos

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Related Experiment Videos

Last Updated: Mar 17, 2026

Live Cell Imaging during Mechanical Stretch

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Area of Science:

Cellular dynamics
Biophysics
Computational biology

Background:

Cellular physiological state is complex, involving mechanical, spatial, and temporal properties.
Traditional assays struggle to quantify cell dynamics comprehensively and non-invasively.
Accurate characterization of cell mechanics and behavior is crucial for understanding cellular function.

Purpose of the Study:

To introduce an efficient, non-invasive computational tool for analyzing unlabeled live cells.
To automatically detect, segment, and analyze cell behavior from time-lapse images.
To provide quantitative kinematic, mechanical (stiffness, viscosity), and spatiotemporal parameters.

Main Methods:

Development of a computational tool utilizing time-lapse imaging.
Automatic detection, segmentation, and analysis of live cells without labeling.
Measurement of cellular shape, migration, stiffness, and viscosity.
Application to human mesenchymal stem cells (huMSCs) and T-lymphocyte cells (T cells).

Main Results:

The tool successfully analyzed unlabeled live cells, extracting shape, migration, stiffness, and viscosity parameters.
Detected significant differences in cellular stiffness between huMSCs and osteogenically induced huOBs.
Distinguished between naïve and stimulated T cells based on mechanical and dynamic properties.
Results for cellular stiffness were comparable to atomic force microscopy findings.

Conclusions:

The developed computational tool offers an integrated approach to deciphering cell dynamics.
Provides a novel, non-invasive method for comprehensive cell characterization.
Enables detailed analysis of spatiotemporal and intracellular dynamics, advancing cell biology research.