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Related Experiment Video

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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

A compact fiber-optic probe-based singlet oxygen luminescence detection system.

Nathan R Gemmell¹, Aongus McCarthy², Michele M Kim³

¹Division of Electronic and Nanoscale Engineering, University of Glasgow, UK.

Journal of Biophotonics

|July 26, 2016

Summary

This study introduces a new fiberoptic probe for detecting singlet oxygen near-infrared luminescence. The advanced system significantly improves signal quality for potential in vivo tissue applications.

Keywords:

fluorescence photon counting quantum optics singlet oxygen

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Area of Science:

Biomedical Optics
Photochemistry
Photonics

Background:

Singlet oxygen (¹O₂) plays a crucial role in photodynamic therapy and biological processes.
Accurate detection of ¹O₂ luminescence is essential for understanding its role in biological systems.
Existing detection methods face challenges with background noise and sensitivity.

Purpose of the Study:

To develop a novel, compact fiberoptic probe for sensitive singlet oxygen near-infrared luminescence detection.
To improve signal-to-noise ratio (SNR) for luminescence measurements.
To investigate the feasibility of in vivo applications by examining light scattering effects.

Main Methods:

Utilized a fiberoptic probe coupled to an InGaAs/InP single photon avalanche diode (SPAD) detector.
Implemented patterned time gating to minimize dark counts and photosensitizer background luminescence.
Employed spectral filtering and lifetime fitting for luminescence detection at 1270 nm.
Investigated the impact of light scattering on signal detection.

Main Results:

Achieved singlet oxygen luminescence detection at 1270 nm using Rose Bengal and Photofrin as model photosensitizers.
Demonstrated a 50-fold improvement in SNR compared to previous fiberoptic-coupled superconducting nanowire single-photon detector systems.
Successfully characterized the effect of light scattering, paving the way for in vivo studies.

Conclusions:

The developed fiberoptic-based SPAD system offers a highly sensitive and robust platform for singlet oxygen luminescence detection.
The significant SNR enhancement overcomes limitations of prior systems.
This technology holds promise for advancing in vivo diagnostics and research in photodynamic applications.