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Updated: Mar 17, 2026

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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A mitochondrial-derived vesicle HOPS to endolysosomes using Syntaxin-17

Gábor Juhász1

  • 1Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, H-6726 Hungary Department of Anatomy, Cell, and Developmental Biology, Eötvös Loránd University, Budapest, H-1117 Hungary szmrt@elte.hu.

The Journal of Cell Biology
|July 27, 2016
PubMed

Insights

Damaged mitochondria are packaged into vesicles for removal. The study reveals Syntaxin-17 protein mediates the fusion of these mitochondrial-derived vesicles with endolysosomes for cellular degradation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Organelle Biology

Background:

  • Damaged mitochondria are packaged into mitochondrial-derived vesicles (MDVs) for degradation.
  • The precise mechanism targeting MDVs for degradation remains unclear.

Purpose of the Study:

  • To elucidate the mechanism by which mitochondrial-derived vesicles (MDVs) are targeted for degradation.
  • To identify the molecular players involved in MDV trafficking and fusion.

Main Methods:

  • Investigated the role of SNARE proteins in MDV trafficking.
  • Utilized cell-based assays to track MDV-endolysosome interactions.
  • Examined the localization and function of Syntaxin-17 in relation to MDVs.

Main Results:

  • The SNARE protein Syntaxin-17 was identified as a key mediator in the process.
  • Syntaxin-17 facilitates the fusion of MDVs with endolysosomes.
  • This fusion event is crucial for delivering mitochondrial components to lysosomes.

Conclusions:

  • Syntaxin-17 plays a critical role in the degradation pathway of damaged mitochondria via MDVs.
  • The findings clarify a key step in mitochondrial quality control and cellular waste removal.

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