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Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics
Jia-You Fang1,2, Pei-Wen Wang3, Chun-Hsun Huang4
1Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan.
Proteomics
|July 28, 2016
Summary
This study reveals distinct molecular changes in skin aging. UVB exposure causes DNA damage and inflammation, while natural aging impairs cell growth and increases protein damage.
Area of Science:
- Dermatology
- Molecular Biology
- Proteomics
Background:
- Skin aging involves intrinsic (chronological) and extrinsic (e.g., UVB) factors.
- Aging alters skin structure, molecular characteristics, and biochemical processes, impairing its protective functions.
Purpose of the Study:
- To investigate and compare specific molecular responses in nude mouse skin during aging induced by different factors.
- To identify key proteins and molecular pathways involved in intrinsic and extrinsic skin aging.
Main Methods:
- Immunohistological investigation of mouse skin.
- Proteome profiling to analyze protein expression.
- Network analysis to understand molecular interactions.
Main Results:
- UVB exposure led to increased proliferating cell nuclear antigen and DNA oxidative damage.
- Chronological aging resulted in reduced epidermal cell growth, increased caspase-14, and protein carbonylation.
- Network analysis suggested ubiquitin system activation and downregulation of 14-3-3 sigma in programmed aging, potentially accelerating cell growth loss.
- UVB stimulation was linked to enhanced inflammation and increased skin carcinogenesis risk.
Conclusions:
- Functional proteomics is a powerful tool for large-scale investigation of aging-related skin proteins.
- Distinct molecular mechanisms underlie UVB-induced and chronological skin aging.
- Understanding these pathways can inform strategies to mitigate aging-related skin damage and disease risk.
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