Related Experiment Video

Updated: Jul 6, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

MicroRNA Profiling from RSV-Infected Biofluids, Whole Blood, and Tissue Samples

Lydia Anderson¹, Patricia A Jorquera¹, Ralph A Tripp²

¹Animal Health Research Center, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 29, 2016

Abstract:

Several studies have shown that respiratory syncytial virus (RSV) can modulate the host innate immune response by dysregulation of host microRNAs (miRNAs) related to the antiviral response, a feature that also affects the memory immune response to RSV (Thornburg et al. MBio 3(6), 2012). miRNAs are small, endogenous, noncoding RNAs that function in posttranscriptional gene regulation. Here, we explain a compilation of methods for the purification, quantification, and characterization of miRNA expression profiles in biofluids, whole blood samples, and tissue samples obtained from in vivo studies. In addition, this chapter describes methods for the isolation of exosomal miRNA populations. Understanding alterations in miRNA expression profiles and identifying miRNA targets genes, and their contribution to the pathogenesis of RSV, may help elucidate novel mechanism of host-virus interaction (Rossi et al., Pediatr Pulmonol, 2015).

Keywords:

Exosomes MicroRNAs RNA isolation RT-qPCR Respiratory syncytial virus

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

Articles linked to this work by shared authors, journal, and citation graph.

Label-free surface-enhanced Raman spectroscopy and machine learning for rapid classification and quantification of bovine respiratory disease complex viruses.

Talanta·2026

Probenecid is a dual-acting antiviral/anti-inflammatory therapy for diverse respiratory viruses.

Frontiers in pharmacology·2026

Intersection of inflammation and viral replication: the central role of MAPK signaling in viral respiratory infections.

Frontiers in microbiology·2026

Evaluation of Probenecid Against Filovirus Replication in Vero E6 Cells.

Viruses·2026

Probenecid Treatment Inhibits Replication of the Edmonston Measles Virus Strain in Vero Cells.

Viruses·2025

Restricting O-Linked Glycosylation of the Mucin-like Domains Enhances Immunogenicity and Protective Efficacy of a Respiratory Syncytial Virus G Glycoprotein Vaccine Antigen.

Vaccines·2025

See all related articles