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Updated: Mar 17, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
Published on: September 23, 2021
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy
Bin-Bin Pan1, Feng Yang1, Yansheng Ye2
1State Key Laboratory and Research Institute of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. xunchegnsu@nankai.edu.cn.
Abstract:
Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.
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