Jove
Visualize
Contact Us

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

960
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
960
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.7K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fourier-basis active structured illumination sensing lidar imaging using crossed acousto-optic devices.

Applied optics·2026
Same author

Time-shifted pseudo-noise multibeam lidar array using acousto-optic deflectors.

Applied optics·2025
Same author

Terahertz imaging using optically controlled Fourier-basis structured illumination.

Applied optics·2022
Same author

Monopulse ladar: super-resolved 3D localization with Si-photonic serpentine optical phased arrays.

Applied optics·2022
Same author

Vernier optical phased array lidar transceivers.

Optics express·2022
Same author

Fourier-basis structured illumination imaging with an array of integrated optical phased arrays.

Journal of the Optical Society of America. A, Optics, image science, and vision·2021
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 17, 2026

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.8K

Single-shot afocal three-dimensional microscopy.

Daniel Feldkhun, Kelvin H Wagner

    Optics Letters
    |July 30, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Fourier-basis agile structured illumination sensing (F-BASIS) enables high-speed, focus-free 3D microscopy by capturing dense Fourier samples without scanning. This novel technique achieves volumetric measurements in under 10 milliseconds, ideal for dynamic biological processes.

    More Related Videos

    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
    12:59

    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

    Published on: February 28, 2021

    4.2K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    12.1K

    Related Experiment Videos

    Last Updated: Mar 17, 2026

    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
    06:33

    Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

    Published on: October 29, 2019

    10.8K
    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
    12:59

    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

    Published on: February 28, 2021

    4.2K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    12.1K

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Microscopy

    Background:

    • Traditional microscopy techniques often face limitations in speed and volumetric data acquisition.
    • Capturing fast dynamic biological processes in 3D requires advanced imaging modalities.

    Purpose of the Study:

    • To introduce and demonstrate Fourier-basis agile structured illumination sensing (F-BASIS) for high-speed, focus-free 3D volume microscopy.
    • To showcase the capability of F-BASIS for rapid volumetric measurements.

    Main Methods:

    • Utilizing acousto-optically synthesized moving interference patterns.
    • Employing sparse RF-encoded aperture synthesis and nonredundant spatiotemporal frequency multiplexing.
    • Implementing single-pixel detection for dense 3D Fourier sample acquisition without scanning.

    Main Results:

    • Demonstrated 3D fluorescence imaging using F-BASIS.
    • Achieved unprecedented wide-field single-shot volumetric measurement in under 10 milliseconds.
    • Successfully acquired dense clouds of 3D Fourier samples rapidly and efficiently.

    Conclusions:

    • F-BASIS offers a transformative approach to high-speed 3D imaging.
    • The technology is well-suited for capturing fleeting dynamic processes, such as 3D neuron signaling.
    • F-BASIS overcomes limitations of conventional scanning-based microscopy for volumetric imaging.