Release studies of trans-anethole from β-cyclodextrin solid inclusion complexes by Multiple Headspace Extraction.

Area of Science:

• Food Science and Technology
• Materials Science
• Physical Chemistry

Background:

• Beta-cyclodextrin (β-CD) is widely used for encapsulating active compounds.
• Controlling aroma release from solid inclusion complexes (IC) is crucial for product stability and sensory experience.
• Understanding the influence of environmental factors on aroma release is essential for optimizing formulation and storage.

Purpose of the Study:

• To evaluate the impact of preparation methods (freeze-drying and co-precipitation coupled to freeze-drying) on β-CD/trans-anethole (β-CD/AN) inclusion complex (IC) properties.
• To investigate the effect of temperature and relative humidity (RH) on the release kinetics of trans-anethole (AN) from β-CD/AN IC.
• To determine the optimal conditions for retaining aroma within the IC structure.

Main Methods:

• Preparation of β-CD/AN IC using freeze-drying (FD) and co-precipitation coupled to FD (Cop-FD).
• Multiple headspace extraction-gas chromatography (MHE) for quantifying loading capacity (LC) and encapsulation efficiency (EE%).
• Controlled release studies at various temperatures and RH levels, analyzing release kinetics with Avrami's equation.

Main Results:

• Quantification of encapsulated AN by MHE necessitates IC dissolution.
• Trans-anethole (AN) release rate significantly increases with higher RH and temperature.
• AN release was minimal below 75% RH.
• Cop-FD method demonstrated superior AN retention compared to FD.
• Avrami's equation effectively modeled the AN release behavior.

Conclusions:

• The preparation method is a critical factor influencing aroma retention and release from β-CD IC.
• Environmental conditions (temperature and RH) directly affect the rate of aroma release.
• The Cop-FD method offers enhanced stability for AN encapsulation.
• Selection of the preparation method should be tailored to specific application requirements for aroma control.