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Updated: Aug 17, 2026

Electricity-Free, Sequential Nucleic Acid and Protein Isolation
Published on: May 15, 2012
[Staphylococcal enterotoxin type E: isolation, purification, identification]
Abstract:
Homogeneous protein of staphylococcal enterotoxin type E has been isolated. The technique of isolation, permitting 48% yield of active material, includes concentration by ammonium sulfate precipitation, ion exchange chromatography on DEAE-cellulose and gel-filtration on sephacryl S-200. The molecular mass of the isolated protein is 32 Kd. Antigenic affinity of staphylococcal toxins types A and E has been established by immunochemical analysis.
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