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[Peroxidases and ethylene formation in olive tree leaves]
B Vioque1, J C Fernández-Maculet, M A Albi
1Departamento de Fisiología y Tecnología Postrecolección, Instituto de la Grasa y sus Derivados, Sevilla, España.
Summary
Olive leaf sections lose intercellular peroxidases and their ability to convert 1-aminocyclopropane-1-carboxylic acid (ACC) into ethylene with age. This suggests a link between peroxidase activity and ethylene production in olive trees.
Area of Science:
- Plant Physiology
- Biochemistry
Background:
- Ethylene biosynthesis is crucial for plant development and stress responses.
- Intercellular peroxidases play roles in plant defense and metabolism.
Purpose of the Study:
- To investigate the relationship between intercellular peroxidases and ethylene production in olive leaves.
- To determine how leaf age affects these two processes.
Main Methods:
- Isoelectric focusing of olive leaf sections to assess peroxidase activity.
- Measurement of 1-aminocyclopropane-1-carboxylic acid (ACC) conversion to ethylene.
- Comparison of activities in leaves of different ages.
Main Results:
- Loss of intercellular peroxidases correlated with reduced ACC to ethylene conversion capacity.
- Both activities decreased in parallel with increasing leaf age.
- Inhibition of ethylene production in homogenized leaves was attributed to the release of inhibitors.
Conclusions:
- Intercellular peroxidases are likely involved in the regulation of ethylene biosynthesis in olive leaves.
- Leaf senescence is associated with a decline in both peroxidase activity and ethylene production capacity.