Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Cross-Sectional Street Survey Examining a Preference for Truth-Telling in Serious Illness and End-of-Life in Maputo City Province.

Journal of palliative medicine·2026
Same author

Triage tools to inform the prioritisation of physical health services following a diagnosis of cancer: a scoping review.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2025
Same author

Integrating Positive Psychology and Autism: A Roundtable.

Autism in adulthood·2025
Same author

Evaluating models and assessment techniques for understanding oral biofilm complexity.

MicrobiologyOpen·2023
Same author

Effect of the glucagon-like peptide-1 receptor agonist liraglutide, compared to caloric restriction, on appetite, dietary intake, body fat distribution and cardiometabolic biomarkers: A randomized trial in adults with obesity and prediabetes.

Diabetes, obesity & metabolism·2023
Same author

Factors Associated With Changes in Patient-Centered Care in Undergraduate Nursing Students.

Nursing education perspectives·2023
Same journal

A simple, sensitive microsample LC-MS assay for quercetin and isorhamnetin in mouse and human plasma: application to EMIQ treatment in myotonic dystrophy type 1.

Bioanalysis·2026
Same journal

ADA assays for high-dose biologics: redefining drug tolerance through clinical insights.

Bioanalysis·2026
Same journal

Comparison of SERS spectral data sets of blood serum samples of hypopharyngeal cancer using silver and gold nanoparticles as substrates.

Bioanalysis·2026
Same journal

The Gyrolab platform for immunogenicity assessment and biotherapeutic and biomarker analysis: technical advances and bioanalytical applications.

Bioanalysis·2026
Same journal

Simultaneous quantification of D-penicillamine, D-penicillamine disulfide, and L-cysteine-D-penicillamine disulfide in human plasma: optimization of sample preparation and mass spectrometry procedures to support bioequivalence studies.

Bioanalysis·2026
Same journal

Development and preliminary clinical application of a time-resolved fluoroimmunoassay for anti-rituximab antibodies in membranous nephropathy.

Bioanalysis·2026
See all related articles

Related Experiment Video

Updated: Mar 16, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.6K

Advances in LC: bioanalytical method transfer.

Patricia Wright1, Adrian Wright2

  • 1Keele University Institute of Science & Technology in Medicine, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB UK.

Bioanalysis
|August 6, 2016
PubMed
Summary
This summary is machine-generated.

Scientists are exploring new chromatographic methods to improve bioanalytical assays by balancing speed, cost, and sensitivity. These advancements aim to overcome the trade-offs inherent in traditional methods for better analytical outcomes.

Keywords:
HPLCMSSFCUHPLCbioanalysisselectivitysensitivityvalidation

More Related Videos

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.8K
Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.8K

Related Experiment Videos

Last Updated: Mar 16, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

13.6K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.8K
Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.8K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Analysis

Background:

  • Traditional bioanalytical assays face challenges in balancing speed, cost, and sensitivity.
  • Conventional 4.6-mm id reversed-phase High-Performance Liquid Chromatography (HPLC) methods often require trade-offs between these critical factors.

Purpose of the Study:

  • To review emerging chromatographic technologies for bioanalysis.
  • To evaluate how new methods address the interplay of speed, cost, and sensitivity.
  • To inform bioanalysts about advancements in chromatographic techniques.

Main Methods:

  • Review of recently introduced chromatographic technologies.
  • Comparative analysis of new methods against conventional HPLC.
  • Discussion of factors influencing method selection in bioanalysis.

Main Results:

  • Newer chromatographic technologies offer potential improvements in speed, cost, or sensitivity.
  • Advancements aim to mitigate the traditional trade-offs in bioanalytical method development.
  • Specific technologies provide distinct advantages for bioanalytical applications.

Conclusions:

  • Emerging chromatographic methods present viable alternatives for bioanalytical assays.
  • These technologies can enhance efficiency and performance in bioanalysis.
  • Bioanalysts can leverage these advancements to optimize assay development.