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Inactivation of Pseudomonas aeruginosa by Chitosan Coated Iron Oxide Nanoparticles
Munmun Mukherjee, Sirshendu De1
1Department of Chemical Engineering, Indian Institute of Technology, Kharagpur, Kharagpur - 721302, India.
Background:
Pseudomonas aeruginosa is one of the potent opportunistic pathogens associated with respiratory and urinary tract infection. The bacterium owes its pathogenicity due to the intrinsic resistance to antibiotics and disinfectants.
Objective:
The present study is focused on the synthesis of antibacterial chitosan coated iron oxide nanoparticles for rapid inactivation of Pseudomonas aeruginosa. We have discussed the relevant patents on synthesis and antibacterial potential of metallic nanoparticles and chitosan.
Method:
Chitosan coated iron oxide nanoparticles were synthesized by coprecipitation method at room temperature using non-toxic chitosan and iron salts in alkali media. The particles were characterized and evaluated for antibacterial property against Pseudomonas aeruginosa.
Results:
The average size of the particles was measured as 52 nm. The surface area of the coated particles was as high as 90 ±5 m2/g. FTIR spectra confirmed the coating of chitosan on nanoparticles. The coated particles showed excellent antibacterial activity against the bacteria. The minimum inhibitory concentration of the coated particles was 105)µg mol-1. The morphological alteration and cytoplasmic leakage of bacteria were confirmed by SEM image and release of intracellular constituents, respectively. Higher 260 nm absorbance value confirmed stronger antibacterial activity of the coated nanoparticles as compared to pure chitosan and bare iron oxide nanoparticles.
Conclusion:
The study indicated that chitosan coated iron oxide nanoparticles have superior antibacterial property as compared to pure chitosan and iron oxide nanoparticles.
Insights
Chitosan coated iron oxide nanoparticles effectively inactivate Pseudomonas aeruginosa. These nanoparticles demonstrate superior antibacterial activity compared to pure chitosan or iron oxide alone, offering a promising solution for combating resistant bacteria.
Area of Science:
- Materials Science
- Nanotechnology
- Microbiology
Background:
- Pseudomonas aeruginosa is a resilient opportunistic pathogen causing significant respiratory and urinary tract infections.
- Its pathogenicity is amplified by inherent resistance to conventional antibiotics and disinfectants.
Purpose of the Study:
- Synthesize antibacterial chitosan-coated iron oxide nanoparticles for rapid Pseudomonas aeruginosa inactivation.
- Review existing patents on metallic nanoparticle and chitosan synthesis and their antibacterial applications.
Main Methods:
- Chitosan-coated iron oxide nanoparticles synthesized via coprecipitation at room temperature.
- Utilized non-toxic chitosan and iron salts in an alkaline medium.
- Characterized nanoparticles and evaluated antibacterial efficacy against Pseudomonas aeruginosa.
Main Results:
- Synthesized nanoparticles averaged 52 nm with a high surface area (90 ±5 m²/g).
- FTIR confirmed successful chitosan coating.
- Demonstrated excellent antibacterial activity against Pseudomonas aeruginosa, with a Minimum Inhibitory Concentration (MIC) of 105 µg/mL.
- SEM imaging revealed morphological damage and cytoplasmic leakage in bacteria, indicating potent antibacterial action.
Conclusions:
- Chitosan-coated iron oxide nanoparticles exhibit significantly enhanced antibacterial properties compared to bare iron oxide nanoparticles and pure chitosan.
- The developed nanoparticles present a promising strategy for combating Pseudomonas aeruginosa infections.
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