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Updated: Mar 16, 2026
Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
Published on: November 5, 2010
Fluorescent cyanine dyes for the quantification of low amounts of dsDNA
B B Bruijns1, R M Tiggelaar2, J G E Gardeniers2
1Mesoscale Chemical Systems, MESA(+) Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7500 AE, Enschede, The Netherlands; Life Science, Life Sciences, Engineering & Design, Saxion University of Applied Sciences, M. H. Tromplaan 28, 7513 AB, Enschede, The Netherlands.
Abstract:
In this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal wavelengths for excitation and emission and for each dye the limit of detection and the response linearity at low levels of dsDNA are determined. No linear range was found for SYBR Green and YOYO-1 for pg-ng quantities of dsDNA. EvaGreen, PicoGreen, AccuClear and AccuBlue NextGen show good linearity in the pg-ng range. AccuClear exhibits the widest linear range of 3 pg-200 ng, whereas AccuBlue NextGen turned out to have the highest sensitivity of the tested dyes with a limit of detection of 50 pg.
