One-step competitive lateral flow biosensor running on an independent quantification system for smart phones based
Nan Cheng1, Yuancong Xu1, Kunlun Huang2
1Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Abstract:
In this study, a one-step lateral flow biosensor (LFB) has been developed, optimized and validated for quantitative detection of Hg(II) in water. In the measurement principle, just one T-rich ssDNA probe (TSP) for the specific binding process was successfully employed in the competitive LFB based methods. The concept of an independent quantification system was realized using a cresol red dot as an external standard, which effectively eliminates false negative results. Under optimized conditions, the limit of detection for Hg(II) was 4nM; high selectivity towards Hg(II) and extraordinary device-to-device repeatability of the LFB were achieved. Furthermore, Hg(II) from tap water samples was analyzed, and the results were confirmed by ICP-MS. The interference from other components in the real samples could be neglected during the analysis. The approach provides a simple, sensitive, and practical tool for the detection of trace Hg(II) in tap water, showing great promise for in-situ applications.
