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Comprehensive two-dimensional liquid chromatography for polyphenol analysis in foodstuffs
Francesco Cacciola1, Sara Farnetti2, Paola Dugo3,4,5
1Dipartimento di "Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali,", University of Messina, Messina, Italy.
Journal of Separation Science
|August 12, 2016
Summary
Comprehensive two-dimensional liquid chromatography (2D-LC) enhances polyphenol analysis in complex food samples. This technique offers superior separation power compared to traditional methods, aiding in the identification and quantification of these valuable plant compounds.
Area of Science:
- Analytical Chemistry
- Food Science
- Natural Product Chemistry
Background:
- Polyphenols, plant secondary metabolites, exhibit diverse pharmacological effects.
- Structural variability and limited reference materials complicate polyphenol identification in food.
- One-dimensional liquid chromatography (1D-LC) coupled with mass spectrometry (MS) is common but insufficient for complex matrices.
Purpose of the Study:
- To review applications of comprehensive two-dimensional liquid chromatography (2D-LC) for polyphenol analysis in real-world food samples.
- To highlight the advantages of 2D-LC over 1D-LC for complex sample matrices.
Main Methods:
- Review of scientific literature focusing on 2D-LC applications for polyphenol analysis.
- Emphasis on separation science principles and mass spectrometry detection.
- Exclusion of non-food matrix applications.
Main Results:
- Comprehensive 2D-LC provides significantly higher peak capacity and resolution than 1D-LC.
- This enhanced separation power is crucial for analyzing complex mixtures of polyphenols in food.
- 2D-LC facilitates more accurate tentative identification and quantification of trace polyphenols.
Conclusions:
- Comprehensive 2D-LC is a powerful technique for overcoming the challenges in polyphenol analysis of complex food samples.
- It offers superior resolving power essential for detailed characterization of these bioactive compounds.
- The review underscores the impact of 2D-LC in advancing food analysis and natural product research.
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