Development of garlic bioactive compounds analytical methodology based on liquid phase microextraction using response...

Area of Science:

• Analytical Chemistry
• Food Chemistry
• Biochemistry

Background:

• Organosulphur compounds (OSCs) in garlic (Allium sativum L.) exhibit significant biological properties.
• Quantifying OSCs in food matrices and biological fluids is vital for functional food research.
• Accurate determination of OSCs is essential for understanding their health benefits.

Purpose of the Study:

• To develop and validate a novel dispersive liquid-liquid microextraction (DLLME) method coupled with High-Performance Liquid Chromatography-Ultraviolet detection (HPLC-UV).
• To establish an efficient and reliable technique for the extraction and determination of specific OSCs in diverse matrices.
• To optimize the DLLME parameters for enhanced sensitivity and accuracy in OSC analysis.

Main Methods:

• Dispersive liquid-liquid microextraction (DLLME) was employed for sample preparation.
• High-Performance Liquid Chromatography with Ultraviolet detection (HPLC-UV) was used for compound separation and quantification.
• Experimental design and desirability function were utilized to optimize DLLME parameters, identifying chloroform as the optimal extraction solvent and acetonitrile as the dispersant.

Main Results:

• The optimized DLLME-HPLC-UV method demonstrated reliability, precision, and accuracy.
• The method successfully extracted and determined target OSCs including allicin, (E)- and (Z)-ajoene, 2-vinyl-4H-1,2-dithiin (2-VD), diallyl sulphide (DAS), and diallyl disulphide (DADS).
• The methodology was successfully applied to analyze OSCs in cooked garlic, blood plasma, and digestive fluids.

Conclusions:

• The developed DLLME-HPLC-UV method provides an effective tool for quantifying OSCs in complex matrices.
• This novel approach supports advancements in functional food research and the study of garlic's biological properties.
• The method's successful application to real-world samples highlights its practical utility in food analysis and biomedical research.